ELISA: Best Practices and Troubleshooting


Enzyme-linked immunosorbent assay (ELISA) is an antibody-based reaction that allows the detection and quantitation of numerous analytes with the same basic assay process. The reaction uses a solid surface, typically a microplate, to immobilize the analyte. Once immobilized, unwanted materials can be removed by washing and the remaining analyte quantitated. The assay technology uses antibodies in conjunction with enzyme-catalyzed amplification to provide high specificity and high sensitivity. Here we will describe the basic process steps of ELISA, best laboratory practices, along with troubleshooting tips for common problems.  In addition, some of the devices and instrumentation used with this assay technology will be described.

About the presenter

Dr. Paul Held - Applications Laboratory Manager, BioTek Instruments
Dr. Paul Held joined BioTek more than 20 years ago as a research scientist, and more recently became the Applications Laboratory Manager. His responsibilities include managing the day to day operations of BioTek's busy applications laboratory, as well as writing application notes that highlight the myriad applications for BioTek instrumentation. Dr. Held holds a BS degree in Pharmacology, an MS degree in Pathology and Molecular Biology and a Ph.D. in Molecular Biology.

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