We're looking forward to seeing you at AACR! Be sure to stop by Booth #1857 to learn what's new at BioTek Instruments. We also hope to see you at our Exhibitor Spotlight Tutorial and poster presentation - see the details of these below.
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BioTek's Top Cancer Research Application Notes
- Kinetic Proliferation Assay Using Label-Free Cell Counting
Direct label-free high contrast brightfield cell counting capabilities of the Cytation™ 5 Cell Imaging Multi-Mode Reader coupled with cell-handling abilities of the BioSpa™ 8 Automated Incubator provided a fully automated system to conduct an accurate and reproducible long-term proliferation study.
Products: Cytation 5, BioSpa 8
- An Automated Walk-Away System to Perform Differentiation of 3D Mesenchymal Stem Cell Spheroids
The BioSpa™ 8 Automated Incubator integrated with the magnetic adapter on the EL406™ Washer Dispenser automated media exchanges during the cell differentiation process. Brightfield and fluorescent imaging using the Cytation™ 5 Cell Imaging Multi-Mode Reader confirmed the ability of this method to automate differentiation of 3D cultured stem cells.
- Automated Monitoring of Protein Expression and Metastatic Cell Migration using 3D Bioprinted Colorectal Cancer Cells
The Cytation™ 5 Cell Imaging Multi-Mode Reader and Gen5 Microplate Reader and Imager Software provided an easy-to-use, robust system to quantify protein expression and label-free 3D metastatic cell migration.
- Automated Imaging and Dual-Mask Analysis of γH2AX Foci to Determine DNA Damage on an Individual Cell Basis
The Cytation™ 5 Cell Imaging Multi-Mode Reader automated γ-H2AX assay workflow and Gen5 software’s dual-mask capabilities assessed real-time population-level data as well as individual foci data, for a complete data set. This automated assay format provides an accurate, robust method to assess DNA damage in mammalian cells.
- Automated Comet Assay Imaging and Dual-Mask Analysis to Determine DNA Damage on an Individual Comet Basis
High-density 96-well comet slides were imaged in the Cytation™ 5 Cell Imaging Multi-Mode Reader. Gen5’s dual-mask capability linked comet head and tail data per comet so real time population-level data and individual comet data were available for percent DNA in the tail and comet tail moment calculations.
- Performance of a Label-Free Image-Based 2D Scratch Wound Healing Assay to monitor Cell Migration and its Inhibition
Wound closure was monitored using the Lionheart™ FX Automated Live Cell Imager and cellular analysis of the time lapse images was automatically performed using Gen5 software. The results were verified by using an independent second analyzing software provided by MetaVi Labs.
- Multiplexed Detection of Cytokine Cancer Biomarkers using Fluorescence RNA In Situ Hybridization and Cellular Imaging
The Cytation™ 5 Cell Imaging Multi-Mode Reader’s capacity of four fluorescence imaging cubes allowed multiplexed image collection of one to four mRNA targets simultaneously--in single cells, and at a single transcript detection level. RNA expression levels were then determined using Gen5 software’s cellular analysis algorithms.
- Automation of HTRF® elF4E Kinase Assay Using a 3D Tumoroid- Based Cell Model
The MultiFlo™ FX Multi-Mode Dispenser performed the necessary dispense and aspiration steps to complete the HTRF eIF4E assay using the RAFT™ 3D cell culture system. Z' validation data demonstrated the robustness of the automated assay procedure.
- Combining Kinetic Ligand Binding and 3D Tumor Invasion Technologies to Assess Drug Residence Time and Anti-metastatic Effects of CXCR4 Inhibitors
The Synergy™ Neo 2 was used to determine the drug residence time of GPCR ligand inhibitors. The Cytation™ 5 was then used to perform kinetic label-free imaging of 3D spheroid invasion into a surrounding Matrigel matrix. Comparisons were made to determine whether a possible link existed between increasing residence time and inhibition of metastatic invasion.
- Quantification of MMP Activity and Inhibition in a 3D Tumor Invasion Model
The Cytation™ 5 Cell Imaging Multi-Mode Reader was used to automate imaging of a 3D tumor invasion process incorporating spheroids embedded in a Matrigel matrix, and measure signal generation following cleavage of the selective MMP substrate. Gen5 Data Analysis Software allowed accurate quantification of invasion, in addition to MMP activity, in both the original spheroid and invadopodia.
- Imaging and Analysis of a High Density Cell Migration Assay
The Cytation™ 5 and Gen5 software provided an automated method for quantifying a migration assay using the Oris™ Pro system in 384-well format. Gen5 Image+ software provided robust assessment of cell migration into the cell-free zone of the Oris™ Pro plates using either percent confluence or closure as readouts.
- Live Cell Imaging of GPCR Dependent Second-Messenger Systems
The Cytation™ 3 Cell Imaging Multi-Mode Reader rapidly imaged and analyzed genetically encoded fluorescent biosensors to a number of GPCR related second messengers. Four separate imaging channels allowed for multiplexed fluorescence imaging at 4x and 20x magnification.
A Combined, Affordable Solution for the Performance of Automated 3D in vitro Hepatotoxicity Testing
Tuesday, April 4
1:00 - 5:00 PM
Partners: Hamilton, Reprocell, n3D Biosciences, Inc., UTHealth
Featured Products at AACR
Exhibitor Spotlight Tutorial:
Demonstration of a High Throughput, Image-Based 2D and 3D Natural Killer Cell Mediated Cytotoxicity Assays
Tuesday, April 4
4:00 - 5:00 PM
Exhibitor Spotlight Theater B
In this tutorial we will describe a novel 3D NK cell mediated cytotoxicity assay. Target cancer cells were embedded within a collagen hydrogel and propagated to create tumoroids. Cancer and NK cells were labeled with individual tracking dyes, followed by NK cell addition. Fluorescent apoptosis and necrosis probes were also added to track cytotoxic events within the tumoroids. Kinetic cellular imaging and analysis was performed to monitor NK cell:tumoroid binding and induced target cell apoptosis and necrosis. Experimental testing validated that the combined assay technique provides a sensitive, accurate, and repeatable in vitro method to determine the ability of NK cells to target and kill tumor cells.