We're looking forward to seeing you at AACR! Be sure to stop by Booth #1857 to learn what's new at BioTek Instruments. We also hope to see you at our Exhibitor Spotlight Tutorial and poster presentation - see the details of these below.

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BioSpa 8 Automated Incubator

BioTek's Top Cancer Research Application Notes

Poster Presentation:
 

A Combined, Affordable Solution for the Performance of Automated 3D in vitro Hepatotoxicity Testing


Tuesday, April 4
1:00 - 5:00 PM
Partners: Hamilton, Reprocell, n3D Biosciences, Inc., UTHealth
Poster: #4213


Automated dual-mask analysis. (A) Primary mask placed around the entire spheroid, and secondary mask placed around discontinuous areas of statistically increased GFP signal in (B) untreated; and (C) compound treated spheroids.

Featured Products at AACR

MultiFlo™ FX
Multi-Mode Dispenser

Lionheart™ FX
Automated Microscope

Cytation™ 5
Imaging Reader

 

Exhibitor Spotlight Tutorial:

Demonstration of a High Throughput, Image-Based 2D and 3D Natural Killer Cell Mediated Cytotoxicity Assays 

Tuesday, April 4
4:00 - 5:00 PM
Exhibitor Spotlight Theater B

 


In this tutorial we will describe a novel 3D NK cell mediated cytotoxicity assay. Target cancer cells were embedded within a collagen hydrogel and propagated to create tumoroids. Cancer and NK cells were labeled with individual tracking dyes, followed by NK cell addition. Fluorescent apoptosis and necrosis probes were also added to track cytotoxic events within the tumoroids. Kinetic cellular imaging and analysis was performed to monitor NK cell:tumoroid binding and induced target cell apoptosis and necrosis. Experimental testing validated that the combined assay technique provides a sensitive, accurate, and repeatable in vitro method to determine the ability of NK cells to target and kill tumor cells.